The Effect of Cell-Free DNA from Blood Serum of Mice with Metastatic Melanoma on Enhancement of Oncogenic Properties of Melanoma Cells

Abstract Currently, a significant increase in the levels of circulating cell-free DNA (cfDNA) blood patients is considered as generally recognized marker development oncological diseases. Although tumor-associated cfDNA has been well studied, its biological functions remain unclear. In this work, we investigated effect isolated from serum mice with B16-F10 metastatic melanoma on properties cells vitro . It was found that profile differs significantly healthy mice, and similar to genomic B16 regards abundance oncogenes mobile genetic elements (MGE). shown penetrated into cells, resulting MGE fragments, caused 5-fold mRNA level secreted DNase Dnase1l3 slight Jun , Fos Ras Myc oncogenes. intracellular regulatory EndoG 4-fold oncogenes, which are well-known triggers large number signal cascades, apoptosis inhibition increased tumor cell proliferation. Thus, it obvious origin able penetrate and, despite fact no changes were viability migration activity cfDNA, even single exposure, can cause at cellular oncogenicity recipient cells.